3D culture models:
We have developed 3D scaffold-free tissue models for bone and cartilage studies;
【Bone】
- Induction of osteocyte differentiation in 3D culture models using osteoblasts or MSCs.
【Cartilage】
- Induction of the initial stage of endochondral ossification using 3D chondrocyte spheroid culture
- Kim, Tomida et al, Biotechnol Bioeng, 2022 (link)
Biomechanical studies:
- Measuring the mechanical properties of 3D culture models
- Ongoing
- Measurement of the nuclear morphology in 3D culture models
- Inagaki, Kim et al, Integr Biol, 2023 (link)
- Evaluation of the multicellular behaviors of 3D culture models
- Kim et al, BBRC, 2022 (link)
Mechanobiological studies:
- Elucidation of the effect of hydrostatic pressure on osteocytic spheroids
- Kim, Niioka et al, in submission.
- Comparative gene expression analysis by modulation of cell-cell interaction and cell-substrate interaction
- Kim et al, J Biosci Bioeng, 2021 (link)
- Elucidating the effect of external stimulation on cell and tissue models
Funded projects:
【Principal Investigator】
The JSPS Grant-in-Aid for Young Scientists
Elucidation of osteocyte differentiation mechanism for establishment of bone organoids
(¥4,680,000; 2023 – 2025)
The Foundation of Public Interest of Tatematsu Research Grant (A1)
Reconstruction of bone organoids and elucidation of osteocyte differentiation mechanism
(¥3,000,000; 2022 – 2024)
The Nagoya – Melbourne Joint Research Workshops Fund 2024
Emerging technologies to study biomechanical and mechanobiological characterization of bone, cartilage, muscle and tendon
PIs: Jeonghyun Kim (Nagoya Univ) & Kathryn Stok (Univ of Melbourne)($AUD5,000 for Jeonghyun Kim & $AUD15,000 for Kathryn Stok; Dec 2024)
【Co-Investigator】
The JSPS Grant-in-Aid for Scientific Research (B)
TBA
PI: Eijiro Maeda
CIs: Takeo Matsumoto, Jeonghyun Kim
(2024 – 2027)
The JSPS Grant-in-Aid for Scientific Research (A)
Morphogenesis-based manufacturing: Construction of optimal structures based on mechanical response of cells
PI: Takeo Matsumoto
CIs: Eijiro Maeda, Jeonghyun Kim
(2021 – 2024)
Previous projects:
【Principal Investigator】
The Nagoya – Melbourne Joint Research Workshops Fund 2023
Emerging technologies to study biomechanical and mechanobiological characterization of bone, cartilage, muscle and tendon
PIs: Jeonghyun Kim (Nagoya Univ) & Kathryn Stok (Univ of Melbourne)($AUD15,000 for Jeonghyun Kim & $AUD5,000 for Kathryn Stok; Sep 2023)
The CCI Holdings Co., Ltd. Research Grant
Development of hydrostatic pressurizing device to elucidate the osteocyte mechanotransduction
(¥1,000,000; 2023 – 2024)
The Hitachi Global Foundation (the Kurata Grant)
Practical application of bone regenerative medicine: Construction of 3D pellet culture model using pluripotent stem cells
(¥1,000,000; 2023 – 2024)
The Naito Science and Engineering Foundation Research Grant
Modulation of osteocyte differentiation in 3D culture model using hydrostatic pressure
(¥1,000,000; 2022 – 2023)
The Nakatani Foundation for Advancement of Measuring Technologies in Biomedical Engineering, Grant for Biomedical Engineering Research (Encouragement of Research)
Development of 4D measurement method for bone-cartilage formation process using 3D tissue model
(¥4,000,000; 2021 – 2023)
The JSPS Grant-in-Aid for Young Scientists
Reconstruction of three-dimensional osteochondral model by multilayered scaffold-free tissue
(¥4,160,000; 2020 – 2023)
The JSPS Grant-in-Aid for Research Activity Start-up
Establishment of mass-productive fabrication method and new applications for bone organoids reconstructed by rotatory culture toward bone reconstruction
(¥2,860,000; 2019 – 2021)
The JSPS Research Fellowship for Young Scientists (DC2-PD)
Uterine regeneration and development of uterine implantation patch using decellularized matrix and mechanical stimuli
(¥1,940,000; 2017 – 2019)
Cell mechanics group, Biomechanics lab 